Alpha Lifetech Inc. developed a stage of art to promote the development of proteome study by utilizing advanced peptide array and microarray technologies. As one of the excellent bio-manufacturers, we have a mission to provide high-quality services to our customers.
Custom Lysine & Arginine Methyltransferase Peptide Array Service
Protein methylation was initially found in histone and epigenetic research, and currently progress has now identified hundreds of non-histone targets of methyltransferases. The basic methyl transfer reaction is the catalytic attack of a nucleophile (carbon, oxygen, nitrogen, or sulfur) on a methyl group to form methylated derivatives of proteins, lipids, polysaccharides, nucleic acids, and various small molecules. Protein lysine (K) and arginine (R) methyltransferases share a similar catalytic core structure utilizing a common methyl donor, S-adenosylmethionine (AdoMet). Arginine methylation can occur in the mono-methyl, symmetrical di-methyl or asymmetrical di-methyl state. Lysine methyltransferases are a family of proteins that contain a SET (SET (Su (var) 3 - 9, Enhancer of Zeste, Trithorax) domain or non-SET domain. Each lysine amine group can be modified by the addition of one, two or three methyl groups. Recently, a group of enzymes that antagonize or remove histone methylation have been identified. Histone methyltransferase plays important roles in regulating transcription, genome integrity and epigenetic inheritance. Dysfunction of methylation-regulating enzymes in the body has been noted in some types of human cancers, such as liver cancer, suggesting associations between protein methylation and malignant transformation of cells or formation of tumors. In recent years, epigenetic modification of the histone proteins, especially the methylation of the histone H3, has been an emerging area of research in cancer development.
Nowadays, there are more than 2,500 proteins from various species containing more than 5,000 methylation sequences and thus methyltransferase peptide array service that can free scientists from the laborious and time-consuming work by using our custom service and related products. To create an efficient and high throughput screening for the substrates of methyltransferases or demethylases of interest, Alpha Lifetech Inc. deliberately collects the peptide substrates labeled/non-labeled by methyl group in special sites (Arginine and Lysine) from our database of methylated sites and custom methylation or demethylation peptide microarray for our clients, as figure 1 shows: A. Peptide lysine array; B. Peptide arginine array.
-- Lyophilized peptides, 5-50 nmole in each well
-- No cross contamination
-- Quality Control: MALDI-TOF QC and COA
-- Turnaround time: Typically, 2-3 week
Applications of Lys & Arg Methyltransferase Peptide Array
-- Methylation and Demethylation Profiling
-- Identification of methyltransferase/demethylase, using pre-defined substrates
-- Characterization of substrate specificity of a given methyltransferase/demethylase
-- Comparison between/among multiple methyltransferase/demethylase
-- Fingerprinting among/between different cells, tissues
Besides, Alpha Lifetech Inc. also provides various modifications, including Isotope labels, fluorescent dye, phosphorylation, glycosylation, N-Methyl amino acids, O-sulfonation, cyclic peptides, fluorophores, MAPS and Carrier Complex, unusual amino acids, peptide mimetics, etc. Please feel free to contact us to get more information and methylated sites.