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Human primary cells are cells isolated directly from human tissues. The majority of human primary cells are terminally differentiated cells with a highly specific type and high level of function. They closely mimic the physiological state of cells in vivo and are expected to maintain their in vivo functions under optimal conditions for a short period of time.

Human primary cells are established as an important tool for in vitro cell-based assays or for generating in vivo models. The primary cells present several advantages over immortalized cell lines, including a better representation of the cellular heterogeneity of tissues, a more faithful transcriptomic and proteomic profile and more realistic functional responses.

Primary cell culture refers to the culturing of cells directly isolated from tissues or organs. Tissue masses are disaggregated using chemical, mechanical or enzymatic methods to break-up tissues and obtain viable cells. The cells are then induced to grow in culture vessels based on cell characteristics.

 

Figure 1. Primary cell culture from lung tissue

 

Alpha Lifetech Inc. offers hundreds of human primary cells for the study of biological processes, such as studies of cell communication, developmental biology, and the mechanisms in biological processes. Primary cells are isolated from various tissues including aorta, ventricle, kidney, lung, skeletal muscle, skin and myometrium. Classified by cell type, our human primary cell portfolio includes epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, muscle cells, hematopoietic cells, etc.

 

 

We offer cells, optimized growth medium, and everything else you need for your cell culture application. With Alpha Lifetech Inc.'s well-established cell biology platform and molecular biology platform, we guarantee that the human primary cells provided by Alpha Lifetech Inc. are ethically sourced, quality controlled, identified and approved for research use. The source of human primary cells is documented and quality control (QC) testing and cell identification are performed to ensure cells are free of contaminants.  A certificate of analysis is provided for each product purchased.

 In addition, custom products are available for non-standard cell types according to your specific requirements. For more information, please contact us and you will receive technical support as soon as possible.