Cell Line Introduction


A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space. Lines differ from cell strains in that they become immortalized.



Primary Cells


Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. Each mammalian tissue type is derived from the embryonic germ layer consisting of ectoderm, endoderm, and mesoderm, which differentiate into the many cell types that organize into tertiary structures such as skin, muscle, blood and blood vessels, etc. Epithelial cells, fibroblasts, keratinocytes, melanocytes, endothelial cells, muscle cells, hematopoietic cells, and mesenchymal stem cells are the cell types most frequently used in research in primary cell culture.


Stable Cell Lines


Stable cell lines are crucial laboratory tools that can be used to express large amounts of a protein of interest. High protein production may be necessary for the screening of experimental drugs, studying of gene functions, or production of therapeutic proteins (including recombinant antibodies). The cell lines indefinitely reproduce and continue to express the transgene consistently during that period.

Generation of a stable cell line refers to the process of developing homogenous populations of cells that demonstrate expression of a transfected gene insert. The transfected gene integrates into the genome of the host cell, and as a result, can express the transfected genetic material. Stable cell lines contrast strongly with transiently transfected cells, which do not have stable genetic and protein expression characteristics. Transient transfection results in temporary changes to cell lines, which may aid in one-time production of proteins or short-term experiments, but problematic for studies that last for several days or more. As such, genetic modifications of cells used for longer studies must be permanent. 



To accelerate the biological research, Alpha Lifetech Inc. launched over 100+ types of single clone-derived cell lines for cell-based bioassays that overexpressing trending targets, including neonatal Fc receptor, G protein-coupled receptors and Fc gamma receptors. With Alpha Lifetech Inc.'s well established molecular biology platform and cell biology platform, we announced that the stable cell lines provided by Alpha Lifetech Inc. are all quality guaranteed: multiple species are available, single clone of each cell line are promised. All cell lines are passed strictly QC test.

For more information, please contact us and our technical support team will get back to you as soon as possible.



[1] AABU, BPMP. Cell Lines-ScienceDirect[J]. Encyclopedia of Genetics, 2001, 2(1): 310-311.

[2] Wang Y, Song E, Leng Y, et al. The Primary Cell Culture of Rabbit Retinal Müller Cell in Vitro[J]. Journal of Jilin University of (Medicine Edition), 2003.