█ Description
Silver nanoparticles are an ideal alternative to gold nanoparticles due to their absorption maximum in the 400nm range, instead of 500nm for gold nanoparticles. Silver nanoparticles retain the same protein and other ligand binding properties of gold nanoparticles.
These products are suitable for conjugating proteins and DNA using standard EDC/NHS coupling chemistry.
Our silver nanoparticles are available in different sizes ranging from 10-100nm, and are synthesized using a unique protocol. Our synthesis method produces monodisperse particles with a narrow and uniform size distribution (CV<15%).
For custom sizes, formulations or bulk quantities please contact our customer service department.
█ Product Information
|
Type |
Silver nanoparticles |
|
Diameter |
10nm-100nm |
|
Surface |
NHS, Carboxyl, Biotin (or others) |
|
Absorbance (λmax) |
390-490nm |
|
Concentration |
~ 0.02 mg/ml (or others) |
|
Nr of functionalized groups on surface |
~ 1/nm2 |
|
Size |
0.5ml, 1ml, 5ml, 10ml, 20ml (or others) |
|
Storage |
Stored at 2-8°C. Do not freeze. |
|
Shelf life |
6 months |
█ Application
--Development of peptide and protein silver conjugates
--Blotting
--Lateral flow assays
--LSPR assays
--SERS
--Light microscopy
--Dark field
--Transmission electron microscopy (TEM)
|
Diameter (nm) |
Peak SPR Wavelength (nm) |
NPS/ml |
Wt. Conc. (mg/ml) |
Size Dispersity (+/-nm) |
Particle Volume (nm3 ) |
Surface Area (nm2 ) |
Surface/ Volume Ratio |
Particle Mass (g) |
Molar Mass (g/mol) |
Molar Conc. |
|
10 |
390-405 |
~3.6E+12 |
2.00E-02 |
<18% |
5.24E+02 |
3.14E+02 |
0.6 |
5.49E-18 |
3.31E+06 |
5.98E-09 |
|
20 |
390-410 |
~4.6E+11 |
2.00E-02 |
<15% |
4.19E+03 |
1.26E+03 |
0.3 |
4.39E-17 |
2.65E+07 |
7.64E-10 |
|
30 |
400-410 |
~1.4E+11 |
2.00E-02 |
<15% |
1.41E+04 |
2.83E+03 |
0.2 |
1.48E-16 |
8.93E+07 |
2.32E-10 |
|
40 |
405-425 |
~5.7E+10 |
2.00E-02 |
<15% |
3.35E+04 |
5.03E+03 |
0.5 |
3.52E-16 |
2.12E+08 |
9.47E-11 |
|
50 |
410-430 |
~2.9E+10 |
2.00E-02 |
<12% |
6.54E+04 |
7.85E+03 |
0.12 |
6.87E-16 |
4.13E+08 |
4.28E-11 |
|
60 |
425-450 |
~1.7E+10 |
2.00E-02 |
1.13E+05 |
1.13E+04 |
0.1 |
1.19E-15 |
7.14E+08 |
2.82E-11 |
|
|
80 |
440-480 |
~7.1E+09 |
2.00E-02 |
<12% |
2.68E+05 |
2.01E+04 |
0.075 |
2.81E-15 |
1.69E+09 |
1.18E-11 |
|
100 |
480-520 |
~3.6E+09 |
2.00E-02 |
<10% |
5.24E+05 |
3.14E+04 |
0.06 |
5.49E-15 |
3.31E+09 |
5.98E-12 |
█ Handling
When stored for a long period of time silver nanoparticles may sediment at the bottom of the flask, which is especially true for larger particle sizes. Prior to use, re-suspend the sedimented particles by swirling until a homogenous solution is obtained.
|
Size (nm) |
Speed (g) |
Time (min) |
|
10 |
21,000 |
60 |
|
20 |
17,000 |
30 |
|
30 |
11,000 |
30 |
|
40 |
3,000 |
30 |
|
50 |
1,800 |
30 |
|
60 |
900 |
30 |
|
80 |
500-600 |
30 |
|
100 |
300 |
30 |
█ Preparation of Silver Conjugate
1. Transfer the desired volume of silver nanoparticles to 1.5ml Eppendorf tubes.
2. Add Tween 20 to a final concentration of 0.025% (w/v).
3. Centrifuge the solution to pellet the silver nanoparticles. For more information on appropriate centrifugation settings for silver nanoparticles of different sizes, see Handling.
4. Resuspend the silver nanoparticles with 2mM sodium citrate to the original silver colloid volume and concentration.
5. Adjust the pH of the silver nanoparticle solution as determined in the titration procedure above.
6. Add the appropriate amount of protein as determined in the titration procedure above plus an additional 10%.
7. Incubate for 60 minutes at room temperature on a rotary shaker/rocker.
8. Centrifuge the vial for 30 minutes at the appropriate speed for the silver nanoparticlesize that you are conjugating to pellet the particles and remove the supernatant.
9. Resuspend the pellet in 1X PBS supplemented with 1% BSA (w/v). Sonicate briefly in a sonicator bath to aid in dispersion if particles are partially agglomerated.
10. Store the silver conjugate at 4 degrees Celsius until use.
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