Monoclonal Antibody Production by Hybridoma

Hybridoma, a hybrid cell produced by the fusion of two kinds of cells, namely mouse myeloma cell line and plasma cells (lymphocyte B), which the heterozygous cell can produce continuously antibody against the antigen of interest in vitro. So far as, scientists have successfully developed the techniques for the construction of several kinds of hybridoma cell lines including murine (mouse, rat, guinea pig and hamster) and rabbits.

The production of a hybridoma cell line consists of three parts: antigen design (haptens, small molecules and peptides), animal immunization, cell fusion and positive clone screening. Utilizing unique immunization methodologies, we obtain exceptionally high cell-fusion efficiencies (1-10 hybridomas per thousand B cells) and high antibody titers, maximizing our success rate in subsequent analytical tests that include biochemical screening, in-vitro cell-based assays and animal studies. Alpha Lifetech Inc. can provide epitope prediction, peptide synthesize, protein expression, polyclonal or monoclonal antibody production as well as antibody purification from cell culture, ascites fluid or whole serum. The processes of the production of monoclonal antibodies:

Please check the detailed items as below to find the best method of production of monoclonal antibody to fit your project: