Alpha Lifetech Inc. can provide quality assured custom polyclonal antibody (pAbs) services including the customized protocol for rabbit immunization, serum-collection and titer analysis for rabbit antibody production, plus extension possibilities.
Antibody Production Service - Custom Rabbit Polyclonal Antibody Production
Rabbit polyclonal antibodies (pAbs) against the human antigens often have a low background signal in ELISA assay, especially it was used to measure the number of viral antigens in vitro. In addition, rabbit pAbs are commonly used as detection antibodies in immunochemistry staining (IHC) of pathology.
Cytokeratin 7 (CK7): rabbit anti-CK7 polyclonal antibody (1: 800)
We offer the scientists one-stop support for all steps of rabbit polyclonal antibody production: from peptide design, synthesis and carrier protein conjugation to animal immunization, serum collection, titer analysis, and final antibody purification. We have two platforms, namely peptide synthesis and protein expression platform, and specialize in antigen design and generation of custom peptide antibodies.
The general workflow of custom rabbit polyclonal antibody is shown below:
Antigen Preparation: protocol design, antigen design & synthesis, hapten/small molecules modification (KLH, BSA, OVA, Hydralink-conjugated), other antigens like viruses, bacterium and whole cells preparation
Antibody Production: 3 - 4 boost immunity, serum titer detection by ELISA and bleed
Antibody Purification: affinity purification (protein A/G), ion exchange and peptide affinity (ligand affinity purification) (optional).
Antibody Modification (optional): enzymes (HRP or AP), fluorescence groups, agarose bead, quantum dot, magnetic bead conjugation etc.
Service Features of Custom Rabbit Polyclonal Antibody Production
-- High-quality rabbit polyclonal antibodies to tailor your requirements
-- Excellent document support system and strict QC & QA
-- Guarantee rabbit antiserum titer: > 50, 000
-- Excellent choice for IHC assay
-- Excellent choice for development of antibodies against small molecules or haptens
-- Multiple strategies are available for immunization: 28, 42, 72, 90 and 120-day (ELISA for each checkpoint)
If you have any questions about the selection of antibody in your project, please don't hesitate to discuss with our antibody experts to get some advice and contact us at any time.