GPCR Memebrane Protein Service

Alpha Lifetech Inc. focuses on recombinant proteins' expression in different biosystems for many years, including prokaryotic and eukaryotic organism, and also for the development of novel high-potency recombinant proteins to meet your technical, program, and budget requirements.

Membrane Proteins & Cell lines - G Protein-Coupled Receptor Expression Service

G-protein-coupled receptors (GPCRs), also called G protein–linked receptors (GPLR) or 7TM receptors, are the largest and most diverse group of membrane receptors in eukaryotes including yeast, choanoflagellates and animals. So far as, one-third known genes in human cells code membrane proteins in which two-third of these membrane proteins are GPCRs or GPCRs related proteins. In fact, researchers estimate that between one-third and one-half of all marketed small molecules act by binding to GPCRs.

Figure 1. 2-D structure of GPCR: Seven-transmembrane domain; C (1-4) and E (1-4) loops interact with signaling molecules inside or outside cell matrix


Figure 1 shows that a single polypeptide folds into GPCR and seven segments of this molecule span the entire width of the membrane embed in a cell's plasma membrane. While the 3-D structure of GPCRs look like a globular shape and the extracellular loops form part of the pockets at which signaling molecules bind to the GPCR.


Figure 2. 3-D structure of GPCRs: Endogenous agonist bounds to the seven-transmembrane domain which changes the conformation of GPCR and then actives cellular signaling pathway


GPCRs need to combine other subunits (α, β, γ subunit) in order to play a whole function. G protein (α subunit) possesses GTPase activity which can hydrolyse GTP and then provide energy for this molecular machine to transform the conformation of GPCRs, show in figure 3:

Figure 3. Actives cellular signaling pathway induced by GPCRs


To produce functional and native GPCRs membrane proteins, Alpha Lifetech Inc. has developed a powerful Cell/Cell-free Expression platform based on different organisms including bacteria, plant and mammal cells. Alpha Lifetech Inc. can express recombinant proteins, especially membrane proteins, in a form of precipitation without detergents or a solubilized form in 24 detergents in cell-free systems, according to different applications and requirements of each project to meet the need of our clients.