Alpha Lifetech Inc. is experienced in providing customers with various stable cell line construction services. We are committed to offering an advanced custom cell line ervicr to each of you and our services & products are proven and trusted.
Stable Cell lines - Genome Editing Cell Lines Generation Service
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system, which is an important component of bacterial adaptive immunity, consists of the Cas nuclease and two individual RNA components, a programmable crRNA (CRISPR RNA) and a fixed tracrRNA (trans-activating crRNA). Cas protein can cut the invading phage DNA into small fragments, which are then integrated into the CRISPR array as a spacer. Subsequently, the CRISPR array is transcribed to generate crRNA and a complementary tracrRNA, which form a double-stranded RNA structure that recruits Cas proteins for cleavage. Adjacent to the crRNA-targeted sequence on the invading DNA, a short sequence named the protospacer adjacent motif (PAM) plays an essential role in the adaptation and interference stages, which the CRISPR/Cas complex recognizes during target DNA binding. The absence of a PAM sequence can alter the affinity between the Cas9 protein and the target DNA, since a specific PAM sequence serves to recognize target sequences and discriminate others from non-target sequences.
CRISPR/Cas9 system, one of the CRISPR/Cas systems, requires only one cas9 protein, which functions in both RNA-guided DNA recognition relying on sgRNA (single guide RNA) formed by crRNA and tracrRNA fusion and cleavage, making this system potentially useful for genome engineering applications. Alpha Lifetech Inc. uses a modified CRISPR/Cas9 system, where modification of Cas9 protein based on its functional domains, to serve each project with individual genome-editing requirements and provide relative stable cell lines in a short turnaround time. The modified Cas9 protein can generate a double-strand break (DSB) at the targeted region, and then activate the DNA repair mechanism to induce insertions or deletions of the gene of interest.
Program Features of Genome Editing Cell Lines Generation Service
-- High efficiency: Precise genomic editing, high knock-out/knock-in efficiency
-- High success rate: Extensive species spectrum, no species restrictions
-- Fast turnaround：10 - 14 weeks
-- Multiple tasks: multiple gene sites can be operated simultaneously
-- Excellent for CAR-T gene editing
Service Procedures of Genome Editing Cell Lines Generation Service
In addition, we can offer you the gene knock down/in animal models based on our CRISPR/CAS9 technology platform and SPF animal feeding facility, by which we can generate susceptible animal models, including patient-derived xenograft (PDX) and cell line-derived xenograft (CDX) mouse models.
If you have any questions, please feel free to contact us at any time.