█ Description

Magnetic particles are functionalized with various terminal active groups for further conjugation of affinity ligands. The conjugation between particles and ligands can be established by direct conjugation, click chemistry, and linker chemistry. In direct conjugation stratagem, magnetic beads surface can be functionalized with amine, carboxyl, sulfhydryl, aldehyde, and active hydrogen functional groups. In "click" chemistry, magnetic beads are functionalized with alkyne to react with azide functionalized ligands under Cu catalyst. Conjugation by linker chemistry offers control over the molecular orientation of bound ligands, which is critical for protecting ligands functionality. Magnetic beads surface functionalized with amine or carboxyl groups can be modified with heterobifunctional linker molecules and further reacted with ligands.

█ Product Information

█ Application

--Medical diagnostics

--Catalysis

--Probing agents to indirectly study the structure of mixed SAMs

--Solid phase extraction

--Anode materials for Li-ion batteries

--Electromagnetic interference (EMI) shielding materials.

█ Coupling method

1. Take an appropriate amount of magnetic beads and wash them several times with pH8.0 buffer solution. Disperse an appropriate amount of biomolecules in a pH 8.0buffer solution, and re-disperse the magnetic beads with this solution. Couple them at 25°C for 3 hours or at 4°C overnight.

2. After coupling, magnetically attract the beads, discard the supernatant. Add 1% BSA-containing buffer, re-disperse the beads. Couple at 25°C for 1 hour to block unreacted NHS groups.

3. After Blocking, magnetically attract the beads. Remove the supernatant, wash the beads 3 times with 20 mM pH 7.4 PBS buffer, and redisperse them. Store at 4°C for later use. If necessary, add 0.02% sodium azide to inhibit bacterial growth.