█ Description
Non-Functionalized Standard Gold Nanoparticles are classical citrate coated gold nanoparticles. These particles can be used for adsorption of proteins or modification and functionalization with thiolated ligands such as PEGs and oligonucleotides.
These products are suitable for conjugating proteins and DNA using standard EDC/NHS coupling chemistry.
Our Gold nanoparticles are available in different sizes ranging from 5 -400nm, are more than 95% spherical and have uniform size distribution (CV <12%).
For custom sizes, formulations or bulk quantities please contact our customer service department.
█ Product Information
|
Type |
Gold nanoparticles |
|
Diameter |
5nm-400nm |
|
Surface |
Amine, Carboxyl, Biotin (or others) |
|
Absorbance (λmax) |
510-570nm |
|
Concentration |
~ 0.05 mg/ml (or others) |
|
Nr of functionalized groups on surface |
~ 1/nm2 |
|
Size |
0.5ml, 1ml, 5ml, 10ml, 20ml (or others) |
|
Storage |
Stored at 2-8°C. Do not freeze. |
|
Shelf life |
6 months |
█ Application
--Development of gold conjugates
--Blotting
--Lateral flow assays
--LSPR assays
--Light microscopy
--Transmission electron microscopy (TEM)
|
Diameter (nm) |
Peak SPR Wavelength (nm) |
NPS/ml |
Wt. Conc. (mg/ml) |
Molar Ext (M-1cm-1) |
Size Dispersity (+/-nm) |
Particle Volume (nm3 ) |
Surface Area (nm2 ) |
Surface/ Volume Ratio |
Particle Mass (g) |
Molar Mass (g/mol) |
Molar Conc. |
|
5 |
515-520 |
5.74E+13 |
6.94E-02 |
1.10E+07 |
<15% |
6.54E+01 |
7.85E+01 |
1.2 |
1.27E-18 |
7.64E+05 |
9.08E-08 |
|
10 |
515-520 |
5.98E+12 |
6.07E-02 |
1.01E+08 |
<15% |
5.24E+02 |
3.14E+02 |
0.6 |
1.02E-17 |
6.11E+06 |
9.93E-09 |
|
15 |
520 |
1.64E+12 |
5.61E-02 |
3.67E+08 |
<12% |
1.77E+03 |
7.07E+02 |
0.4 |
3.43E-17 |
2.06E+07 |
2.72E-09 |
|
20 |
524 |
6.54E+11 |
5.31E-02 |
9.21E+08 |
<12% |
4.19E+03 |
1.26E+03 |
0.3 |
8.12E-17 |
4.89E+07 |
1.09E-09 |
|
30 |
526 |
1.79E+11 |
4.91E-02 |
3.36E+09 |
<12% |
1.41E+04 |
2.83E+03 |
0.2 |
2.74E-16 |
1.65E+08 |
2.98E-10 |
|
40 |
530 |
7.15E+10 |
4.65E-02 |
8.42E+09 |
<12% |
3.35E+04 |
5.03E+03 |
0.5 |
6.50E-16 |
3.91E+08 |
1.19E-10 |
|
50 |
535 |
3.51E+10 |
4.45E-02 |
1.72E+10 |
<10% |
6.54E+04 |
7.85E+03 |
0.12 |
1.27E-15 |
7.64E+08 |
5.83E-11 |
|
60 |
540 |
1.96E+10 |
4.30E-02 |
3.07E+10 |
<10% |
1.13E+05 |
1.13E+04 |
0.1 |
2.19E-15 |
1.32E+09 |
3.25E-11 |
|
70 |
548 |
1.20E+10 |
4.17E-02 |
5.03E+10 |
<10% |
1.80E+05 |
1.54E+04 |
0.086 |
3.48E-15 |
2.10E+09 |
1.99E-11 |
|
80 |
553 |
7.82E+09 |
4.06E-02 |
7.70E+10 |
<10% |
2.68E+05 |
2.01E+04 |
0.075 |
5.20E-15 |
3.13E+09 |
1.30E-11 |
|
90 |
564 |
5.37E+09 |
3.97E-02 |
1.12E+11 |
<8% |
3.82E+05 |
2.54E+04 |
0.067 |
7.40E-15 |
4.46E+09 |
8.92E-12 |
|
100 |
572 |
3.84E+09 |
3.89E-02 |
1.57E+11 |
<8% |
5.24E+05 |
3.14E+04 |
0.06 |
1.02E-14 |
6.11E+09 |
6.37E-12 |
|
150 |
|
3.60E+09 |
1.20E-01 |
|
<8% |
1.77E+06 |
7.07E+04 |
0.04 |
3.43E-14 |
2.06E+10 |
5.98E-12 |
|
200 |
|
1.91E+09 |
1.55E-01 |
|
<8% |
4.19E+06 |
1.26E+05 |
0.03 |
8.12E-14 |
4.89E+10 |
3.17E-12 |
|
250 |
|
7.08E+08 |
1.13E-01 |
|
<8% |
8.18E+06 |
1.96E+05 |
0.024 |
1.59E-13 |
9.55E+10 |
1.18E-12 |
|
300 |
|
4.50E+08 |
1.24E-01 |
|
<8% |
1.41E+07 |
2.83E+05 |
0.02 |
2.74E-13 |
1.65E+11 |
7.48E-13 |
|
400 |
|
1.88E+08 |
1.22E-01 |
|
<8% |
3.35E+07 |
5.03E+05 |
0.015 |
6.50E-13 |
3.91E+11 |
3.12E-13 |
█ Handling
When stored for a long period of time gold nanoparticles may sediment at the bottom of the vial, which is especially true for larger particle sizes. Prior to use, re-suspend the sedimented particles by swirling until a homogenous solution is obtained.
|
Size (nm) |
Speed (g) |
Time (min) |
|
5 |
100,000 |
30 |
|
10 |
17,000 |
60 |
|
15 |
17,000 |
30 |
|
20 |
6,500 |
30 |
|
30 |
4,500 |
30 |
|
40 |
2,500 |
30 |
|
50 |
2,000 |
30 |
|
60 |
1,125 |
30 |
|
80 |
600 |
30 |
|
100 |
400 |
30 |
|
150 |
180 |
30 |
|
200 |
100 |
30 |
█ Preparation of Gold Conjugate
1. Transfer amount of gold nanoparticles needed for your application from the stock toa new tube.
2. Add protein amount as determined above plus an additional 10%.
3. Incubate for 30 minutes at room temperature while stirring.
4. Centrifuge the solution for 30 minutes at the appropriate speed for the gold nanoparticle size used. For more information on appropriate centrifugation speeds of gold nanoparticles of different sizes, see Gold Nanoparticle Handling and Storage.
5. Resuspend the pellet in PBS supplemented with 0.1% BSA or 1% PEG.
6. Store at 4°C until use.
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