A variety of methods are currently in use for the fast and efficient generation of target-specific nanobodies. Single-domain antibodies have many advantages: High affinity with small size antibody, good physicochemical properties and downstream engineering easily. The characteristics of single domain and ease of gene manipulation of the single domain antibody (sdAb) make it suitable for affinity maturation in vitro.
As an excellent provider in the field of phage display and antibody engineering, Alpha Lifetech Inc. offers single domain antibodies (sdAbs) affinity maturation services as part of our end-to-end antibody development services.
Phage Display Library – Antibody Affinity Maturation for Single Domain Antibodies Service
Although single domain antibodies (sdAbs) isolated from antibody libraries derived from gene synthesis and immunized or naïve animals generally exhibit reasonable affinity, it is often desirable to further increase their affinity. Quantitative library sorting by phage display is used for improving the affinity of single-domain antibodies. We have developed a proprietary DNA mutagenesis technique that can create a huge number (e.g. 1010) variants of the parental antibody with defined positions mutated. In combination with our screening technologies including Alanine Peptide Scanning Library, Overlapping Peptide Library, Positional Peptide Library, Scrambled Peptide Library and Peptide Array Screening, 10-100-fold increase in affinity for parental single domain antibodies of low nM affinity is frequently achieved. This is often possible for other companies for antibodies of 100nM affinity or worse.
Alpha Lifetech Inc. is proud to offer comprehensive library construction services to meet the diverse needs of global researchers. We aim to understand and meet the demands of different customers and to assist with any upcoming and emerging problems in research work.
Please feel free to contact us at any time, our sales representative or technical support manager will prepare a detailed quote accordingly.