Alpha Lifetech Inc. is capable of providing different species or different isotypes of native or synthesized Fab antibody libraries based on our mature phage display technology platform. Building up a high-quality antibody library with a large library size and high diversity has been crucial for the successful isolation of antibodies, it will result in the speed of antibody-drug discovery. Now Alpha Lifetech Inc.'s antibody discovery R&D team is proud to announce that we can develop a synthesized Fab library with independent clones over 1011.
Phage Display Library – Fab Antibody Library Construction Service
The IgG molecule can be subdivided into two functional subunits: (1) the fragment crystallizable (Fc), which constitutes the tail of the antibody and interacts with cell surface receptors to activate an immune response, and (2) the fragment-antigen binding (Fab), which mediates antigen recognition. The Fc region comprises two pairs of constant domains (CH2 and CH3) from two paired heavy chains, whereas the Fab region consists of a variable domain followed by a constant domain from the heavy chain (VH and CH, respectively), which pair with a variable and constant domain from the light chain (VL and CL, respectively).
Fab Library Construction Service
Fab libraries, in which light-chain (LC) and heavy-chain (HC) variable region genes are cloned into a phagemid vector and subsequently displayed on the surface of the filamentous phage particle, have been widely used for the isolation of antibodies (Abs) with specificity for haptens, foreign antigens (Ags), and self Ags.
Fc Library Construction Service
As we know, the Fc fragment of the antibody can be used as a tag that co-expressed with recombinant protein, and Fc fragments have been successfully used instead of intravenous immunoglobulins. One of the most valuable features of the Fc domain in vivo is that it can dramatically prolong the plasma half-life of the small molecules of interest and result in an improved efficacy that has been mediated by a high-affinity interaction between Fc fragment and Fc receptors. Building up a high-quality antibody library with a large library size and high diversity has been crucial for the successful isolation of antibodies, it will result in the speed of antibody-drug discovery. Now Alpha Lifetech Inc.'s antibody discovery R&D team is proud to announce that we can provide Fab and Fc fragments according to your specific needs.
Alpha Lifetech Inc.'s Fc Library Construction including the following services:
-- Native Fc library Generation Service
-- Synthetic Fc Library Generation Service
-- Fc-chimeric Fusion Protein Library Generation Service
The scientists in Alpha Lifetech Inc. have also modified a vector system that has several features, such as different leader sequences for the light and heavy chains, a stop codon that allows easy shuttling between appropriate host strains for the preparation of phage or the expression of soluble Fab, a Myc tag for analysis and purification of the protein, and a subtilisin cleavage site useful for recovery of bound phage during library screening.
In order to create a larger size antibody library, Alpha Lifetech Inc. utilizes an efficient cloning strategy for the construction of antibody phage libraries based on the isolation of restriction fragments from plasmid vectors instead of PCR products. With a deep understanding of the technology of antibody phage display, we can tailor your project with one-stop construction & screening of antibody libraries, including Single Domain Antibody Library Construction Service, assist customers with any upcoming and problems in library construction and screening.
Alpha Lifetech Inc. is proud to offer comprehensive library construction services to meet the diverse needs of global researchers. Our aim is to understand and meet the demands of different customers and to assist with any upcoming and emerging problems in research work.
Please feel free to contact us at any time, our sales representative or technical support manager will prepare a detailed quote accordingly.