Antibody humanization is used for reducing the immunogenicity of animal monoclonal antibodies (mAbs) and for improving their activities in the human immune system. Our scientists have optimized the traditional algorithm of the CDRs grafting technique, a combination of the advantages of our empirical antibody humanization approaches and traditional algorithms, we can efficiently improve the stability and affinity of humanized antibodies in the body. We identify the CDRs from your hybridoma cell line or antibody sequence (parental antibody), and graft these CDRs into framework regions carefully selected from databases of hundreds of thousands of mature human antibodies. The framework regions of the human immunoglobulin are selected based on a number of factors including homology and position of key residues.

Alpha Lifetech Inc. can offer the antibody affinity maturation service by using mutation and selection technologies. We usually use scFv as the antibody format in the processes of affinity maturation service and a monovalent display phagemid system which has been used to reduce the avidity effects during antigen-binding screening. Our professional team can provide affinity maturation services for single-domain antibodies as well.