Product Datasheet
|
Catalog Number |
ATCS1003 |
|
Product Name |
5637 |
|
Species |
Homo sapiens (Human) |
|
Source |
Established from the primary bladder carcinoma of a 68-year-old man in 1974; cells were described in the literature to produce several growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.). |
|
Type |
Urinary bladder carcinoma |
|
Morphology |
Epithelial |
|
Culture Medium |
90% RPMI-1640 + 10% FBS |
|
Subculture |
Seed out at about 1-2 x 10^6 cells/80 cm²; Split confluent cultures 1:4 to 1:5 using Trypsin/EDTA (cells should be incubated for 5 to 10 min with Trypsin/EDTA at 37 °C). |
|
Split Ratio |
1:4 to 1:5 |
|
Doubling Time |
24 h |
|
Incubation |
Incubate the cells at 37 °C with 5% CO2. |
|
Frozen Medium |
90% culture medium + 10% DMSO |
|
Biosafety Level |
1 |
|
Size |
1 vial |
|
Quantity |
>5×10^5 cells per vial |
|
Protocol |
|
|
Storage |
Vapor phase of liquid nitrogen |
|
Shipping Condition |
The cancer cell lines are shipped out in dry ice. |
|
Note |
This product is for research use only. |
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