Product Datasheet
|
Catalog Number |
ATCS1006 |
|
Product Name |
22Rv1 |
|
Species |
Homo sapiens (Human) |
|
Source |
Derived from a human prostate carcinoma xenograft (CWR22R) that was serially propagated in nude mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft; the cells produce xenotropic murine leukemia virus-related virus (XMRV) (see risk assessment). |
|
Type |
Prostate carcinoma |
|
Morphology |
Epithelial |
|
Culture Medium |
40% RPMI-1640 + 40% DMEM + 20% FBS |
|
Subculture |
Seed out at about 1-2 x 10^6 cells in a 175 cm² flask; Split confluent culture 1:3 to 1:10 every 3-6 days using Trypsin/EDTA for about 10 min. |
|
Split Ratio |
1:3 to 1:10 |
|
Doubling Time |
40-60 h |
|
Incubation |
Incubate the cells at 37 °C with 5% CO2. |
|
Frozen Medium |
90% culture medium + 10% DMSO |
|
Biosafety Level |
1 |
|
Size |
1 vial |
|
Quantity |
>5×10^5 cells per vial |
|
Protocol |
|
|
Storage |
Vapor phase of liquid nitrogen |
|
Shipping Condition |
The cancer cell lines are shipped out in dry ice. |
|
Note |
This product is for research use only. |
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