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Ordering Information

 

Product Datasheet

Catalog Number

ATCS1013

Product Name

8305C

Species

Homo sapiens (Human)

Source

Established from the primary tumor of a 67-year-old woman with primary thyroid carcinoma (undifferentiated carcinoma, composed of spindle, polygonal and giant cells).

Type

Thyroid carcinoma

Morphology

Large spindle-shaped

Culture Medium

80-90% RPMI-1640 + 10-20% FBS

Subculture

Seed out at about 0.5-1 x 10^6 cells/175 cm²; Split confluent culture 1:5 once a week using Trypsin/EDTA.

Split Ratio

1:2 to 1:5

Doubling Time

55-60 h

Incubation

Incubate the cells at 37 °C with 5% CO2.

Frozen Medium

90% culture medium + 10% DMSO

Biosafety Level

1

Size

1 vial

Quantity

>5×10^5 cells per vial

Protocol

Cancer Cell Lines Culture Protocol

Storage

Vapor phase of liquid nitrogen

Shipping Condition

The cancer cell lines are shipped out in dry ice.

Note

This product is for research use only.

 

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