Product Datasheet
|
Catalog Number |
ATCS1016 |
|
Product Name |
A-204 |
|
Species |
Homo sapiens (Human) |
|
Source |
Established from the rhabdomyosarcoma of a 1-year-old girl in 1973; reported to form small malignant tumors in nude mice consistent with embryonic rhabdomyosarcoma. |
|
Type |
Rhabdomyosarcoma |
|
Morphology |
Epithelial |
|
Culture Medium |
85-90% McCoy's 5A medium+ 10-15% FBS |
|
Subculture |
Split confluent culture 1 x per week at up to 1:5 using Trypsin/EDTA; Seed out directly in a 80 cm² culture flask, cells will reach confluency within 5-8 days; cells may clump after Trypsinization and may be difficult to disperse. |
|
Split Ratio |
1:2 to 1:5 |
|
Doubling Time |
36 h |
|
Incubation |
Incubate the cells at 37 °C with 5% CO2. |
|
Frozen Medium |
90% culture medium + 10% DMSO |
|
Biosafety Level |
1 |
|
Size |
1 vial |
|
Quantity |
>5×10^5 cells per vial |
|
Protocol |
|
|
Storage |
Vapor phase of liquid nitrogen |
|
Shipping Condition |
The cancer cell lines are shipped out in dry ice. |
|
Note |
This product is for research use only. |
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