Product Datasheet
|
Catalog Number |
ATCS1018 |
|
Product Name |
A2780 |
|
Species |
Homo sapiens (Human) |
|
Morphology |
Epithelial |
|
Culture Medium |
90% RPMI-1640 modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate + 10% FBS. |
|
Subculture |
Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. T-75 flasks are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains Trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin/EDTA to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. |
|
Split Ratio |
1:2 to 1:5 |
|
Incubation |
Incubate the cells at 37 °C with 5% CO2. |
|
Frozen Medium |
90% culture medium + 10% DMSO |
|
Biosafety Level |
1 |
|
Size |
1 vial |
|
Quantity |
>5×10^5 cells per vial |
|
Protocol |
|
|
Storage |
Vapor phase of liquid nitrogen |
|
Shipping Condition |
The cancer cell lines are shipped out in dry ice. |
|
Note |
This product is for research use only. |
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