Product Datasheet
|
Catalog Number |
ATCS1124 |
|
Product Name |
HeLa |
|
Species |
Homo sapiens (Human) |
|
Source |
Established from the epitheloid cervix carcinoma of a 31-year-old woman in 1951; later diagnosis changed to adenocarcinoma; first aneuploid, continuously cultured human cell line. |
|
Type |
Cervix carcinoma |
|
Morphology |
Epithelial |
|
Culture Medium |
90-95% MEM (with Earle's salts) + 5-10% FBS + 2 mM L-glutamine |
|
Subculture |
Seed out at about 0.5-2 x 10^6 cells/80 cm²; Split confluent culture 1:4 to 1:6 every 3-5 days using Trypsin/EDTA. |
|
Split Ratio |
1:4 to 1:6 |
|
Doubling Time |
40-48 h |
|
Incubation |
Incubate the cells at 37 °C with 5% CO2. |
|
Frozen Medium |
90% culture medium + 10% DMSO |
|
Biosafety Level |
1 |
|
Size |
1 vial |
|
Quantity |
>5×10^5 cells per vial |
|
Protocol |
|
|
Storage |
Vapor phase of liquid nitrogen |
|
Shipping Condition |
The cancer cell lines are shipped out in dry ice. |
|
Note |
This product is for research use only. |
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