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Silver Conjugation Kits typically include all the reagents and protocols necessary for the conjugation of silver nanoparticles (AgNPs) to biomolecules, such as antibodies, proteins, and DNA. The kits may further include purification columns or beads to remove unbound silver nanoparticles (AgNPs) and other impurities. The development of AgNP binding kits enables researchers to  label biomolecules easily with AgNPs.

The conjugation process typically involves the functionalization of the AgNPs with a linker molecule, such as thiolated polyethylene glycol (PEG), followed by the attachment of the biomolecule to the linker. The AgNPs conjugates can then be used for various applications, such as imaging and sensing.


Fig.1 Silver conjugation Kits


Advantages of Silver Conjugation Kits


--Easy to use: The AgNP binding kits is designed to be user-friendly and has a clear scheme, and the kit contains all the necessary reagents. This makes it easy for researchers to label their biomolecules with AgNPs without the need for specialized equipment or expertise.

--High yield: AgNP conjugation kits typically yield high-quality conjugates with minimal loss of biomolecule activity. This ensures that the labeled biomolecules retain their functionality and can be used for various applications.

--Versatility: AgNP conjugation kits can be used to label a wide range of biomolecules, including antibodies, proteins, and DNA. This makes them suitable for various applications in biomedical research.

--Stability: AgNP conjugates are stable and can be stored for long periods of time without losing their activity. This ensures that the labeled biomolecules can be used for multiple experiments and applications.


Applications in Biomedical Research


--Imaging: Silver conjugation kits can be used to label biomolecules with silver nanoparticles for imaging applications. For example, silver-conjugated antibodies can be used for the detection of specific biomarkers in cells and tissues. The silver conjugates can be visualized using various imaging techniques, such as fluorescence microscopy and electron microscopy.

--Drug Delivery: Silver conjugation kits can be used to label drugs with silver nanoparticles, allowing for targeted drug delivery to specific cells or tissues. The silver conjugates can also be used for imaging to track the delivery of the drugs.

--Sensing: Silver conjugation kits can be used to label biomolecules with silver nanoparticles for sensing applications. For example, silver-conjugated DNA probes can be used for the detection of specific nucleic acid sequences in samples. The silver conjugates can be detected using various sensing techniques.


Product Description


The silver conjugation kits provided by Alpha Lifetech Inc. have consistent and reproducible results which are important for researchers who need to produce large quantities of silver conjugates for their experiments.

Alpha Lifetech Inc.'s silver nanoparticle conjugation kits have been optimized for high efficiency one step conjugations of proteins and other primary amine-containing ligands to silver nanoparticles with diameters in the size range of 10nm-100nm. The kit contains pre-made mixtures ready for use. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied in the kit. Kits are available in convenient 3 or 10 small-scale reactions formats allowing multiple to be conjugated simultaneously and ready for use in 2.5 hours or less.

Quality advantage

1. Results in covalently bound ligand and more stable conjugate. 

2. Fast and convenient one-step conjugation reaction.

3. The kits are versatile, sensitive, and reproducible.

Kit Components

1.NHS-Activated Silver Nanoparticles (lyophilized)

2.Protein Re-suspension Buffer

3.Reaction Buffer

4.Quencher Solution


All components of this kit should be stored at -20°C. Activated silver nanoparticles can be stable for at least 3 months if they are unopened and stored according to regulations.